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Course Sample: Handout sent for receipt by participants before the
Student and Teacher Internship Program starts.
* * * * Before the start of the program on Monday, June 23, read:
-- The Safety section and Laboratory #2, pages 317-320,
in DNA Science, A First Course, Second Edition. This is the section on
Laboratory Safety and Adherence to National Institutes of Health Guidelines.
-- Laboratory #2, Bacterial Culture Techniques, Parts
A and B, in DNA Science, pages 331-344.
HHMI/NIH/MCPS
Student
and Teacher Internship Program 2003
Monday, June 23
8:00 a.m. Introductions
• Welcome
from the NIH - (Gloria Seelman, STIP program coordinator)
• Welcome
from DNA Resource Center
• Introduction
of Instructors
Judy
Price, biotechnology teacher/trainer
Patricia
Richards, workshop coordinator
Sanford
Herzon, molecular biology teacher
Karen
Furr, biotechnology manager
• Break
• Pre-Evaluation
of Program
10:00 a.m. Set Up Laboratory Station
Laboratory
Safety
Research
Methods
Prepare
Solutions for DNA Extraction
Lunch
• Laboratory:
DNA Extraction
• Laboratory
#2: Isolation of Colonies,
Inoculate
and Incubate Overnight Culture
3:30 p.m. Review
Homework:
Read
Laboratory #1: Measurements, Micropipetting, and Sterile Techniques, Parts
I and II, in DNA Science, pages 321-328.
Read
Spectrometer Laboratory.
Write
up laboratory protocol in your lab notebook.
Teacher
Credit: Read Chapter 1 in DNA Science, pages 3-35
Tuesday, June 24
8:00 a.m. Laboratory #1: Measurements, Micropipetting and
Sterile Techniques
Laboratory: Measure Concentrations of Solutions using the Spectrometer
20.
Make
a standard curve
Prepare
unknowns
Measure
concentrations
Lunch
• "Proteins"
Video
• Laboratory:
Crystalization of Protein
3:30 p.m. Review
Homework:
Prepare
for ELISA Laboratory
Teacher
Credit: Read Chapter #2 in DNA Science, pages 37-67.
Wednesday, June 25
8:00 a.m. The Basics of Immunology (John Finerty, Ph.D., NIH)
Lunch
1:00 p.m. Laboratory: Use ELISA to Identify the Presence of a Disease
Protein- blotting Techniques (CAT Laboratory, Student Activity) (Vladimir
Knezevic, Ph.D.)
3:30 p.m. Review
Homework:
Read and prepare for Laboratory #5 in DNA Science, pages 395-398
Review DNA structure, replication, transcription and translation
Teacher Credit: Read Chapter 3 in DNA Science, pages 69-105
Thursday, June 26
8:00 a.m. Review DNA Structure, Replication, Transcription and Translation
9:00 a.m. Lecture and Discussion on Plasmids, Probes, Libraries (Suzan
Nadi, Ph.D.)
Lunch
• Laboratory
#5: Rapid Colony Transformation of E.coli with Plasmid DNA
3:30 p.m. Review
Homework:
Read and prepare for Laboratory #3, DNA Restriction Analysis in DNA Science,
pages 351-372
Teacher Credit: Read Chapter 4 in DNA Science, pages 107-140
Friday, June 27
8:00 a.m. Discuss results of Laboratory #5
Prelaboratory:
Gel Electrophoresis
• Laboratory
#3: DNA Restriction Analysis
Lunch
Postlaboratory:
Analysis of Restriction Procedure
3:00 p.m. Laboratory Write-up
Homework:
Write up Laboratory #22 as pre-laboratory preparation
Teacher Credit: Read Chapter 5 in DNA Science, pages 141-182
Teacher Credit: Read Chapter 6 in DNA Science, pages 183-219
Monday, June 30
8:00 a.m. Lecture and Discussion on Polymerase Chain Reaction - Nancy
Sorden, Ph.D. - NIH
• Discussion
- Analysis of Sequencing
Lunch
1:00 p.m. Laboratory #22: Detection of An Alu Insertion Polymorphism by
Polymerase Chain Reaction.
3:00 p.m. Review
Homework:
Read and prepare for Laboratory #8 in DNA Science, pages 423-441.
Prepare overnight for Laboratory #6 moved from the beginning of the day.
Teacher Credit: Read Chapter 7 in DNA Science,
pages 221-258.
Read
Chapter 8 in DNA Science, pages 259-314.
Tuesday, July 1
8:00 a.m. Practical Exam
• Laboratory
#6--Purification and Identification--Practical Exam
• Plasmid
Laboratory
Lunch
• Continue
Plasmid Laboratory
3:00 p.m. Clean-up
Teacher Credit: Take test
Postevaluation of skills
Friday, July 4 -- Day Off (Federal Laboratories Closed for Holiday)
Monday, July 7
8:30 a.m. Brunch in the Cloister with Preceptors and Mentors.
Accompany Preceptors to Laboratory.
Thursday, July 10
1 p.m.-4 p.m. Required Laboratory Safety Course for New Researchers
Building 10, First Floor, Masur Auditorium
Friday, August 15
11:30 a.m.-1:30 p.m. Farewell to Teachers Luncheon in the Cloister Rathskeller
on the Lower Level.
